雾灵山森林公园旅游资源评价

先采用定性方法，从直观上描述了雾灵山森林公园的自然资源、人为景观、旅游环境和开发条件；然后根据游客的调查问卷，又对其进行了定量评价。结果表明：雾灵山的生态旅游资源丰富，区位条件良好，适宜扩大开发生态旅游。并根据评价结果，对公园开发生态旅游提出了几点建议。     

运用数量化方法估测普洱县森林蓄积量

根据普洱县森林遥感调查资料 ,利用卫星影像特征判读数据与地面实测数据建立数学类型 ,估测森林蓄积量。首先将 2 0 0块有、疏林地数据输入数据本中 ,然后分析其与优势树种、龄组、郁闭度、海拔、坡向、坡度的关系 ,结果把样地分为针叶林、阔叶林、针阔混交林三个类型分别建立数学模型 ,以估测每公顷蓄积量。数学模型的建立是根据数量化模型的原理来编程 ,并通过计算机的不断调试 ,直到可行为止。蓄积量比较精度达到 90 3%。     

秭归县退耕还林水源涵养效益计量

林地是森林水源涵养效益的主体,通过对秭归县10种退耕还林模式的土壤物理性状和林地水源涵养效益的研究,结果表明:(1)退耕还林后土壤总孔隙度增加4·3%~20·2%,除柏木林外,非毛管孔隙度增加6·8%~494·2%;(2)退耕还林后土壤饱和蓄水量增加5·7%~118·8%,非毛管蓄水量为坡耕地的2·58~19·13倍;(3)秭归县退耕还林森林土壤的蓄水量为171·6万m3/a,是相似立地条件下农耕地的3·21倍。     

四种桉树青枯菌DNA提取方法及PCR检测灵敏度比较

以带有青枯菌Ralstonia solanacearum的桉树组织为材料,采用4种不同的提取方法抽提青枯菌DNA,同时利用青枯菌的特异性引物进行PCR扩增,比较了4种DNA的提取方法及PCR检测的灵敏度。结果表明,煮沸法和热裂解法操作相对简单,但检测灵敏度较低,检测限分别为104CFU/mL和103CFU/mL;简化提取法和试剂盒法检测效果较好,均可检测到102CFU/mL的青枯菌;简化提取法比试剂盒法操作更简单,检测成本低,具有更高的应用价值。     

新西兰辐射松根朽病与温度、湿度关系的数量模型*

新西兰辐射松根朽病与温度、湿度关系的数量模型束庆龙，宋淑梅，赵卫中，Ｎ．Ｍ．Ｓｅｌｆ关键词辐射松根朽病，温度，降雨量，土壤湿度，数量模型早在１９３０年，新西兰就有关于４年生辐射松ＰｉｎｕｓｒａｄｉａｔａＤ．Ｄｏｎ幼树受根朽病危害致死的报道［１］。６０...     

PCR法克隆紫穗槐4-香豆酸:CoA连接酶全长cDNA

4 香豆酸 :CoA连接酶 (4CL)是木质素生物合成过程中重要的酶 .该文利用简并寡核苷酸PCR法结合cDNA末端快速扩增PCR法直接获得了紫穗槐 4CLAcDNA全长序列 ,避免了复杂的构建、筛选cDNA文库的过程 .先用简并PCR得到了 4CLA1片段 ,又据此片段设计反向嵌套引物 ,用RACE方法获得未知的 5′和 3′端序列 .所获得的全长 4CLAcDNA ,编码 5 40个氨基酸 .氨基酸序列同源性分析表明4CLA是典型的 4CL蛋白 ,含有预计的AMP binding位点、催化反应区和保守的Cys .     

Evaluation of the level of threat and protective classification of the vegetation of Makehe Forest in Sanjiangyuan Nature Reserve, west China

After investigating and studying the vegetation, we have established that Makehe Forest, in Sanjiangyuan Nature Reserve, Qinghai Province, is host to a total of 364 different species, representing 173 “genera” in 55 different vegetative families. We propose five quantifiable indices for evaluating the level of threat to these plants: 1) the distribution frequency of the “line transect”; 2) the distribution frequency of sample plots; 3) the distribution density inside sample plots; 4) the existing abundance in the forest region; and 5) plant fidelity. The results show that there are two endangered species, six vulnerable species, 12 nearly threatened species and 344 safe species. The study tried to evaluate the urgency for conserving these plants, according to coefficients of closeness to disappearance, of genetic loss and of usefulness. Results also showed that the number of species of primary concern is two; of secondary concern, nine; of the third level, 23; and of least concern, 330 species. __________ Translated from Journal of Beijing Forestry University, 2006, 28(3): 20–25 [译自: 北京林业大学学报]     

Comparison of image and rapid field assessments of riparian zone condition in Australian tropical savannas

Suitable methods for measuring and monitoring the condition of riparian environments are being investigated by government agencies responsible for maintaining these environments in Australia. The objective of this work was to compare two riparian condition assessment approaches, the Tropical Rapid Appraisal of Riparian Condition (TRARC) method developed for rapid on-ground assessment of the environmental condition of savanna riparian zones and an image based riparian condition monitoring scheme. Measurements derived from these two approaches were compared and correlated. The sample representativeness of the TRARC method was evaluated and the cost-effectiveness and suitability for multi-temporal analysis of the two approaches were assessed. Two high spatial resolution multi-spectral QuickBird satellite images captured in 2004 and 2005 and coincident field data covering sections of the Daly River in the Northern Territory, Australia were used in this work. Both field and image data were processed to map indicators of riparian zone condition including percentage canopy cover, organic litter on the ground, canopy continuity, tree clearing, bank stability, and flood damage. Spectral vegetation indices, image segmentation, and supervised classification were used to produce riparian health indicator maps. QuickBird image data were used to examine if the spatial distribution of TRARC transects provided a representative sample of ground based estimates of riparian health indicators. Covering approximately 3% of the study area, the sample mean of the TRARC estimates of individual indicators of riparian zone condition were in most cases within 20% of the global mean derived from the whole imaged riparian area. The cost-effectiveness of the image based approach was compared to that of the ground based TRARC method. Results showed that the TRARC method was more cost-effective at spatial scales from 1 km to 200 km of river in relatively homogeneous riparian zones along rivers with only one channel, while image based assessment becomes more feasible at regional scales (200–2000 km of river). A change detection analysis demonstrated that image data can provide detailed information on gradual change, while the TRARC method is less suited for multi-temporal analysis due to the ranked data format, which inhibits precise detection of change. However, results from both methods were considered to complement each other for single date assessment of riparian zones if used at appropriate spatial scales.     

Variation in Heterobasidion annosum detected by Random Amplified Polymorphic DNAs

The production of Random Amplified Polymorphic DNAs (RAPDs) by Polymerase Chain Reaction (PCR) was used to detect variation in the isolates of Heterobasidion annosum with various geographical origins. Specific RAPD products were detected for each of the intersterility groups: the European S, F, P and the North American S group. There was considerably more polymorphism found among European S than P isolates. The analysis of RAPDs was shown to be a simple and a fast way to generate DNA markers specific to the previously established intersterility groups and thus useful for diagnostic purposes.